AmpliTaq and AmpliTaq Gold DNA Polymerase
نویسندگان
چکیده
We report a unique isoform of PLC[beta]4 in rat, PLC[beta]4c, that has an additional 37nucleotide exon inserted between nucleotides 3459-3460 of the previously published PLC[beta]4a coding sequence. This insertion results in replacement of 22 amino acid residues at the carboxyl terminal tail of PLC[beta]4a with 41 unique residues. A human EST for PLC[beta]4 also contains this exon and this exon was mapped to within a 5.5 kb intron of the human PLC[beta]4 gene. PLC[beta]4c is the third PLC[beta]4 isoform to be identified which has a unique carboxyl-terminal tail. PLC[beta]4b differs from PLC[beta]4a by truncation 162 amino acid residues from the carboxyl terminus which are replaced with 10 distinct amino acid residues. Reverse transcription-polymerase chain reaction experiments show that both PLC[beta]4a and PLC[beta]4c mRNA are expressed throughout the rat brain and that PLC[beta]4c mRNA is highly expressed in the eye and cerebellum. RNase protection assays demonstrate that both PLC[beta]4a and PLC[beta]4c transcripts are abundant in the cerebellum. The different carboxyl terminal tails of PLC[beta]4 isoforms may allow for differential targeting and subcellular localization, contributing to regulation of PLC [beta]4-mediated signal transduction.
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An, J. and A. W. Hsie (1993). "Polymerase chain reaction-based deletion screening of bleomycin induced 6-thioguanine-resistant mutants in Chinese hamster ovary cells: The effects of an inhibitor and a mimic of superoxide dismutase." Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 289(2): 215. http://www.sciencedirect.com/science/article/B6T2C-47PCN7M41/2/3157a48d39359d02b0...
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An, J. and A. W. Hsie (1993). "Polymerase chain reaction-based deletion screening of bleomycin induced 6-thioguanine-resistant mutants in Chinese hamster ovary cells: The effects of an inhibitor and a mimic of superoxide dismutase." Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 289(2): 215. http://www.sciencedirect.com/science/article/B6T2C-47PCN7M41/2/3157a48d39359d02b0...
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